Macrófagos en semen humano

BEATRIZ REINA BOUVET, ADRIANA SILVIA BRUFMAN, CECILIA VICENTA PAPARELLA, RODOLFO NESTOR FELDMAN, VANDA NORA GATTI, EDITA AMALIA SOLIS

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Archivos Españoles de Urología ›› 2003, Vol. 56 ›› Issue (9) : 983-987.
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Macrófagos en semen humano

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Abstract

OBJECTIVES: To investigate the presenceof macrophages in human semen samples and the functionthey carry out in the seminal fluid. Their presence wasstudied in relation to spermatic morphology, percentageof spermatozoids with native DNA, and presence ofantispermatic antibodies.METHODS and RESULTS: The work was performedwith semen samples from 31 unfertile males from 63couples in which the "female factor" was ruled out as thecause of infertility. Sperm study according toWHO(1992)was carried out in all samples, in addition to: DNA studywith acridine orange as fluorocrom, macrophage concentration by neutral red in a Neubauer camera, anddetection of antispermatic antibodies with a mixedagglutination test (TAC II) (validated with Mar Screen-Fertility technologies). Sperm morphology was evaluatedby Papanicolaou test.19/31 selectedspermsamples (61.3%)showedincreasedconcentration of macrophages,13 of them (41.9%) withdenaturalized DNA, and 8 (25.8%) abnormal morphology.Six samples showed increased macrophage concentrationand predominance of native DNA, whereas 11 samplesshowedincreasedmacrophagesandabnormalmorphology.Among 18 (58.1%) samples showing antispermaticantibodies 14 (77.7%) had an increased concentration ofmacrophages.Statistical analysis resulted inahigh correlationbetweenmacrophage concentration and increased percentage ofspermatozoids with denaturalized DNA (p < 0.05). Anincreased concentration of macrophages is associatedwith the presence of antispermatic antibodies (p < 0.05).There was not evidence of significant association betweenconcentration of macrophages and percentage ofmorphologically normal spermatozoids (p < 0.5).CONCLUSIONS: We can conclude that macrophagesare present in human semen and participate inimmunovigilance contributing to improve the seminalquality.

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Macrophages / Antisperm antibodies / Spermatic DNA / Sperm morphology / Immunovigilance

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BEATRIZ REINA BOUVET , ADRIANA SILVIA BRUFMAN , CECILIA VICENTA PAPARELLA , RODOLFO NESTOR FELDMAN , VANDA NORA GATTI , EDITA AMALIA SOLIS. Macrófagos en semen humano. Archivos Españoles de Urología. 2003, 56(9): 983-987

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